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The primer sequences used for the qRT-PCR analysis

Journal: Human Cell

Article Title: Adipose-derived stem cells modified by TWIST1 silencing accelerates rat sciatic nerve repair and functional recovery

doi: 10.1007/s13577-024-01087-6

Figure Lengend Snippet: The primer sequences used for the qRT-PCR analysis

Article Snippet: Immunoblots were visualized after incubation with primary antibodies: mouse monoclonal anti-rat TWIST1 antibody (ab50887, Abcam, Cambridge, UK), rabbit monoclonal anti-rat NT-3 antibody (ab263864, Abcam), rabbit monoclonal anti-rat BDNF antibody (ab108319, Abcam), rabbit monoclonal anti-rat NGF antibody (MA5-32067, eBioscience), and mouse monoclonal anti-rat GDNF antibody (sc-13147, Santa Cruz Biotechnology, CA, USA), followed by incubation with secondary antibodies.

Techniques: Sequencing

The mRNA expressions of TWIST1, NT-3, BDNF, NGF, and GDNF in the sciatic nerves of rats after sciatic repair in non-lesioned, non-transplanted, LV-scramble-ADSC, and LV-sh-TWIST1-ADSC groups 8 weeks after transplantation of ADSCs were determined by the qRT-PCR analysis. * P < 0.05 compared to the non-transplanted group and # P < 0.05 compared to the LV-scramble-ADSC group by one-way ANOVA followed by Tukey’s multiple comparisons test

Journal: Human Cell

Article Title: Adipose-derived stem cells modified by TWIST1 silencing accelerates rat sciatic nerve repair and functional recovery

doi: 10.1007/s13577-024-01087-6

Figure Lengend Snippet: The mRNA expressions of TWIST1, NT-3, BDNF, NGF, and GDNF in the sciatic nerves of rats after sciatic repair in non-lesioned, non-transplanted, LV-scramble-ADSC, and LV-sh-TWIST1-ADSC groups 8 weeks after transplantation of ADSCs were determined by the qRT-PCR analysis. * P < 0.05 compared to the non-transplanted group and # P < 0.05 compared to the LV-scramble-ADSC group by one-way ANOVA followed by Tukey’s multiple comparisons test

Article Snippet: Immunoblots were visualized after incubation with primary antibodies: mouse monoclonal anti-rat TWIST1 antibody (ab50887, Abcam, Cambridge, UK), rabbit monoclonal anti-rat NT-3 antibody (ab263864, Abcam), rabbit monoclonal anti-rat BDNF antibody (ab108319, Abcam), rabbit monoclonal anti-rat NGF antibody (MA5-32067, eBioscience), and mouse monoclonal anti-rat GDNF antibody (sc-13147, Santa Cruz Biotechnology, CA, USA), followed by incubation with secondary antibodies.

Techniques: Transplantation Assay, Quantitative RT-PCR

The protein expressions of TWIST1, NT-3, BDNF, NGF, and GDNF in the sciatic nerves of rats after sciatic repair in non-lesioned, non-transplanted, LV-scramble-ADSC, and LV-sh-TWIST1-ADSC groups 8 weeks after transplantation of ADSCs were determined by the immunoblotting analysis. * P < 0.05 compared to the non-transplanted group and # P < 0.05 compared to the LV-scramble-ADSC group by one-way ANOVA followed by Tukey’s multiple comparisons test

Journal: Human Cell

Article Title: Adipose-derived stem cells modified by TWIST1 silencing accelerates rat sciatic nerve repair and functional recovery

doi: 10.1007/s13577-024-01087-6

Figure Lengend Snippet: The protein expressions of TWIST1, NT-3, BDNF, NGF, and GDNF in the sciatic nerves of rats after sciatic repair in non-lesioned, non-transplanted, LV-scramble-ADSC, and LV-sh-TWIST1-ADSC groups 8 weeks after transplantation of ADSCs were determined by the immunoblotting analysis. * P < 0.05 compared to the non-transplanted group and # P < 0.05 compared to the LV-scramble-ADSC group by one-way ANOVA followed by Tukey’s multiple comparisons test

Article Snippet: Immunoblots were visualized after incubation with primary antibodies: mouse monoclonal anti-rat TWIST1 antibody (ab50887, Abcam, Cambridge, UK), rabbit monoclonal anti-rat NT-3 antibody (ab263864, Abcam), rabbit monoclonal anti-rat BDNF antibody (ab108319, Abcam), rabbit monoclonal anti-rat NGF antibody (MA5-32067, eBioscience), and mouse monoclonal anti-rat GDNF antibody (sc-13147, Santa Cruz Biotechnology, CA, USA), followed by incubation with secondary antibodies.

Techniques: Transplantation Assay, Western Blot